Family of Mary, Queen of Scots

King James IV of Scotland
(1473–1513)
Grandfather

Margaret Tudor
(1489–1541)
Grandmother

Claude, Duke of Guise
(1496–1550)
Grandfather

Antoinette de Bourbon
(1493–1583)
Grandmother

James Stewart
Earl of Moray
(1531-1570)
Half-Brother
(illegitimate son of
King James V)

Robert Stewart
1st Earl of Orkney
(1533–1593)
Half-Brother
(illegitimate son of
King James V)

Lady Jean Stewart
(c.1533–1588)
Half-sister

John Stewart
(c.1531–1563)
Half-Brother

King Charles I
(1600-1649)
Grandson

Elizabeth Stuart
Queen of Bohemia
(1596–1662)
Granddaughter

Other Grandchildren:

Margaret (1598–1600)
Robert (1602)
Mary (1605–1607)
Sophia (1607)

Prince Henry
(1594–1612)
Grandson